Flow CAST® Basophil Activation Test

The Flow CAST® is intended for the determination of basophil activation in whole blood samples by flow cytometry. Our unique marker combination – CCR3 and CD63 – combines the simple gating properties for basophil detection by CCR3 with the solid application of the proven activation marker CD63 in one. Thanks to its easy protocol and smart marker combination the first results can be obtained within one hour – perfect conditions for laboratories.

Flow CAST® is for Research Use Only. Not for use in diagnostic procedures

CCR3 as Basophil Marker

CCR3 is detected with a PE-fluorescence labelled monoclonal antibody. This gating of basophil cells is significantly easier than with any other commercially available BAT.

CD63 as Activation Marker

The cell surface marker of basophils, CD63  is detected by a FITC-fluorescence labeled monoclonal antibody. CD63 is currently the best-validated basophil activation marker directly linked to basophil degranulation.

Controls

The first control, anti-FcEpsilonRI, is a highly specific monoclonal antibody binding to IgE receptor. The second control, a tripetide (fMLP) activates the basophils by a non-immunological way.

CD203c as Activation Marker

The CD203c is an other basophil activation marker in addition to the CD63. Our CD203c Reagent Set (RUO) is intended for the use within an extended protocol for the Flow CAST® kit, allowing the measurement of both activation markers, CD63 and CD203c, with two different fluorescence labelled antibodies.
* References available upon request.

Flow CAST® Product Information

Method Flow Cytometry
Time to result 1 h (approx.)
Sample type 50 µl EDTA blood
Marker CCR3-PE / CD63-FITC
Catalog Number see below
Patents US 8,563,258 B2; EP 2 037 269 B1
Status RUO

Flow CAST® + CD203C REAGENT SET Product Information

Method Flow Cytometry
Time to result 1 h (approx.)
Sample type 50 µl EDTA blood
Marker CCR3-PE / CD63-FITC / CD203c-PE-DY647
Catalog Number see below

Downloads and Catalog Numbers

Main applications for Flow CAST®


The CAST® assays can be used for a variety of applications. Further information about specific fields of allergy, ask for independent testing on Cellular Allergy.

PDF Download [EN] (coming soon)

Flow CAST® (Basophil Activation Test) – FK-CCR-U
CD203c Reagent Set – B-CCR-203SET-U

Flow CAST®(Basophil Activation Test) – FK-CCR-U
CD203c Reagent Set – B-CCR-203SET-U

Available in U.S. and Canada.

Discount Available
Discount available on selected products and solutions for short dated products. Please contact us for more information.
US and Canada
Visit our contact page to enter your requests for quotations or to submit an order inquiry.

View Citations
Learn about new publications citing use of our products in a variety of studies, applications, and fields of research.
News and Events
Visit our News page to learn about upcoming events, the latest news about new products, studies and BÜHLMANN in general.
Sign Up for BUHLMANN Updates!

Enter your email and stay on top of things,