The Flow CAST® is intended for the determination of basophil activation in whole blood samples by flow cytometry. Our unique marker combination – CCR3 and CD63 – combines the simple gating properties for basophil detection by CCR3 with the solid application of the proven activation marker CD63 in one. Thanks to its easy protocol and smart marker combination the first results can be obtained within one hour – perfect conditions for laboratories.
Flow CAST® is for Research Use Only. Not for use in diagnostic procedures
CCR3 is detected with a PE-fluorescence labelled monoclonal antibody. This gating of basophil cells is significantly easier than with any other commercially available BAT.
CD63 as Activation Marker
The cell surface marker of basophils, CD63 is detected by a FITC-fluorescence labeled monoclonal antibody. CD63 is currently the best-validated basophil activation marker directly linked to basophil degranulation.
The first control, anti-FcEpsilonRI, is a highly specific monoclonal antibody binding to IgE receptor. The second control, a tripetide (fMLP) activates the basophils by a non-immunological way.
CD203c as Activation Marker
The CD203c is an other basophil activation marker in addition to the CD63. Our CD203c Reagent Set (RUO) is intended for the use within an extended protocol for the Flow CAST® kit, allowing the measurement of both activation markers, CD63 and CD203c, with two different fluorescence labelled antibodies.
* References available upon request.
Flow CAST® Product Information
Time to result
1 h (approx.)
50 µl EDTA blood
CCR3-PE / CD63-FITC
US 8,563,258 B2; EP 2 037 269 B1
Flow CAST® + CD203C REAGENT SET Product Information
BUHLMANN Diagnostics Corp (BDC), Amherst NH is the North American affiliate of BÜHLMANN Laboratories AG, a leading worldwide operative manufacturer of ELISA kits, RIA kits, lateral flow assay kits, flow cytometry assay kits and turbidimetric assays kits. Located in a state-of-the-art facility in Southern New Hampshire, the BDC team collectively represents over 100 years of experience in serving both clinical and basic science research laboratories.