Flow CAST® Highlights

 Flow CAST® is for Research Use Only. Not for use in diagnostic procedures.

One Kit, Two Applications- Accurate Results in Minutes

Flow CAST® is intended for the determination of basophil activation in whole blood samples by flow cytometry. Our unique marker combination (CCR3 and CD63) combines the simple gating properties for basophil detection by CCR3 with the solid application of the proven activation marker CD63 in one test. Thanks to its easy protocol and smart marker combination the first results can be obtained in under an hour which provides perfect conditions for laboratories.


  • Versatile and Robust: Solid applications for allergy testing and for screening in vitro and ex vivo studies
  • Best Standardized Reagents for Basophil Activation: Optimized stimulation buffer, specific activators, and accurate quantification
  • World-Class Support: Gold standard publications, excellent scientific/technical customer support
  • QUICK: 30 minutes to quantified basophil activation inhibition

Kinase Inhibition Application
Simplifying Drug Development

BÜHLMANN’s Flow CAST Assay® for Kinase Inhibition offers a quick and precise means to evaluate inhibitors of immune cell signaling pathway, using inhibition of basophil degranulation as a surrogate read out cell based system by flow cytometry.

Flow CAST Allergy

Allergy Application
Allergy in a vial, resembling in vivo reactions to an allergen  

BÜHLMANN’s Flow CAST ®Assay for Allergy provides an efficient method for cellular allergy stimulation testing using a broad range of quality controlled allergens and mixes which are standardized and validated for use in this assay and the CAST® ELISA.

Learn more about kinase inhibition and check out the Flow CAST® in testing and optimizing kinase inhibitors in drug development.

Flow CAST®  is backed by numerous publications in the food allergy field.  Check out this basophil activation test (BAT) relevant literature list.

Flow CAST® Procedure Steps (Overview)

 Flow CAST® is for Research Use Only. Not for use in diagnostic procedures.

View Product Flyers

Learn more about Flow CAST® in our technical product flyer and take a closer look at pre-analytics as well as assay procedure, specifications, performance and more.

View Technical Flyer

See our full allergen list, product specifications, and ordering details in English and French below.

Learn More

Contact Collin or Jennifer today for more details on Flow CAST® for kinase inhibition and/or allergy.

VP of Business Development

Technical Sales Specialist