MRP8/14 - BUHLMANN - MRP8/14 ELISA - Quantum Blue® MRP8/14

MRP8/14 (serum calprotectin- sCAL)

BÜHLMANN MRP8/14 ELISA and Quantum Blue® MRP8/14 are for Research Use Only. Not for use in diagnostic procedures in the US.

MRP8/14 Assays (myeloid-related protein) (aka serum calprotectin, sCAL, S100A8/S100A9)

The BÜHLMANN MRP8/14 ELISA kit is designed for the quantitative determination of the heteropolymer of myeloidrelated protein MRP8/14 (Calprotectin, S100A8/S100A9) in serum.  The test allows the selective measurement of MRP 8/14- antigen by sandwich ELISA. A monoclonal capture antibody (mAb) highly specific to the MRP8/14 heterodimeric and polymeric complexes respectively, is coated onto the microtiter plate. A second monoclonal detection antibody (Ab) conjugated to horseradish peroxidase (HRP) detects the MRP8/14 molecules bound to the monoclonal antibody coated onto the plate after a washing step. After incubation and a further washing step, tetramethylbenzidine (TMB) will be added (blue color formation) followed by a stopping reaction (change to yellow color). The absorption is measured at 450 nm.

The Quantum Blue®  MRP8/14 lateral flow assay is a sandwich-type immunoassay for the quantitative determination of MRP8/14 in human serum in combination with the BÜHLMANN Quantum Blue® Reader.  The MRP8/14 assays by BÜHLMANN allow for precise and sensitive measurement of this important marker for research related to inflammation.  The test is designed for the selective measurement of MRP8/14 antigen by sandwich immunoassay. A monoclonal capture antibody which is highly specific for MRP8/14 is lined onto the membrane. A second monoclonal detection antibody conjugated to gold colloids is deposited onto the conjugate release pad and released into the reaction system after addition of the diluted serum. The antibody-gold-conjugate reacts with the MRP8/14 of the sample and then binds to the anti-MRP8/14 antibody which is lined onto the membrane (Test Line). The remaining free MRP8/14-gold-conjugate binds to the goat anti-mouse antibody which is lined onto the membrane(Control Line). The signal intensities of the Test Line and the Control Line, respectively, are measured quantitatively by the Quantum Blue® Reader.

Product Information

Name Quantum Blue® sCAL MRP8/14 ELISA
Method Lateral Flow ELISA (96 wells)
Time to result 15 min (approx.) 2 h (approx.)
Sample type 20 μl Serum (1:10) <20 μl Serum/Plasma (1:100)
Standard range 0.5 – 10 μg/mL 0.4 – 24 μg/mL
Sensitivity < 0.5 μg/mL < 0.4 μg/mL
Instructions for Use & Other Downloads see below see below

Download IFU and Catalog Numbers

View the appropriate tab below for your location (US or Canada) and click on the blue underlined catalog number to view the Instructions for Use.

BÜHLMANN sCAL ELISA- EK-MRP8/14-U

Quantum Blue® sCAL- LF-MRP25-U* (coming soon)

* Multilingual [EN, DE, FR, IT, ES]

Quantum Blue® Reader 3rd Generation-
•   BI-POCTR-ABS- Reader User Manual [EN]
•   BI-POCTR-ABS- Reader QC Check Menu [EN]
•    Quick Guide – Fail Safe Mode [EN]
•    Quick Guide – Fast Track Mode [EN]

Quantum Blue® Reader 2nd Generation User Manual-
•   BI-POCTR-ABS- Reader User Manual [EN]
•   BI-POCTR-ABS- Reader User Manual [FR]

BÜHLMANN sCAL ELISA- EK-MRP8/14-U
Quantum Blue® sCAL- LF-MRP25-U* (coming soon)

* Multilingual [EN, DE, FR, IT, ES]

Quantum Blue® Reader 3rd Generation-
•   BI-POCTR-ABS- Reader User Manual [EN]
•   BI-POCTR-ABS- Reader QC Check Menu [EN]
•    Quick Guide – Fail Safe Mode [EN]
•    Quick Guide – Fast Track Mode [EN]

Quantum Blue® Reader 2nd Generation User Manual-
•   BI-POCTR-ABS- Reader User Manual [EN]
•   BI-POCTR-ABS- Reader User Manual [FR]

US and Canada
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