BÜHLMANN MRP8/14 ELISA and Quantum Blue® MRP8/14 are for Research Use Only. Not for use in diagnostic procedures in the US.
MRP8/14 Assays (myeloid-related protein) (aka serum calprotectin, sCAL, S100A8/S100A9)
The BÜHLMANN MRP8/14 ELISA kit is designed for the quantitative determination of the heteropolymer of myeloidrelated protein MRP8/14 (Calprotectin, S100A8/S100A9) in serum. The test allows the selective measurement of MRP 8/14- antigen by sandwich ELISA. A monoclonal capture antibody (mAb) highly specific to the MRP8/14 heterodimeric and polymeric complexes respectively, is coated onto the microtiter plate. A second monoclonal detection antibody (Ab) conjugated to horseradish peroxidase (HRP) detects the MRP8/14 molecules bound to the monoclonal antibody coated onto the plate after a washing step. After incubation and a further washing step, tetramethylbenzidine (TMB) will be added (blue color formation) followed by a stopping reaction (change to yellow color). The absorption is measured at 450 nm.
The Quantum Blue® MRP8/14 lateral flow assay is a sandwich-type immunoassay for the quantitative determination of MRP8/14 in human serum in combination with the BÜHLMANN Quantum Blue® Reader. The MRP8/14 assays by BÜHLMANN allow for precise and sensitive measurement of this important marker for research related to inflammation. The test is designed for the selective measurement of MRP8/14 antigen by sandwich immunoassay. A monoclonal capture antibody which is highly specific for MRP8/14 is lined onto the membrane. A second monoclonal detection antibody conjugated to gold colloids is deposited onto the conjugate release pad and released into the reaction system after addition of the diluted serum. The antibody-gold-conjugate reacts with the MRP8/14 of the sample and then binds to the anti-MRP8/14 antibody which is lined onto the membrane (Test Line). The remaining free MRP8/14-gold-conjugate binds to the goat anti-mouse antibody which is lined onto the membrane(Control Line). The signal intensities of the Test Line and the Control Line, respectively, are measured quantitatively by the Quantum Blue® Reader.
| Name | Quantum Blue® sCAL | MRP8/14 ELISA |
| Method | Lateral Flow | ELISA (96 wells) |
| Time to result | 15 min (approx.) | 2 h (approx.) |
| Sample type | 20 μl Serum (1:10) | <20 μl Serum/Plasma (1:100) |
| Standard range | 0.5 – 10 μg/mL | 0.4 – 24 μg/mL |
| Sensitivity | < 0.5 μg/mL | < 0.4 μg/mL |
| Instructions for Use & Other Downloads | see below | see below |
Download IFU and Catalog Numbers‡BÜHLMANN sCAL ELISA- EK-MRP8/14-U
Quantum Blue® sCAL- LF-MRP25-U* (coming soon)
* Multilingual [EN, DE, FR, IT, ES]
Quantum Blue® Reader 3rd Generation-
• BI-POCTR-ABS- Reader User Manual [EN]
• Quick Guide – Fail Safe Mode [EN]
• Quick Guide – Fast Track Mode [EN]
Quantum Blue® Reader 2nd Generation User Manual-
• BI-POCTR-ABS- Reader User Manual [EN]
• BI-POCTR-ABS- Reader User Manual [FR]
BÜHLMANN sCAL ELISA- EK-MRP8/14-U
Quantum Blue® sCAL- LF-MRP25-U* (coming soon)
* Multilingual [EN, DE, FR, IT, ES]
Quantum Blue® Reader 3rd Generation-
• BI-POCTR-ABS- Reader User Manual [EN]
• Quick Guide – Fail Safe Mode [EN]
• Quick Guide – Fast Track Mode [EN]
Quantum Blue® Reader 2nd Generation User Manual-
• BI-POCTR-ABS- Reader User Manual [EN]
• BI-POCTR-ABS- Reader User Manual [FR]
