Flow CAST^® is for Research Use Only. Not for use in diagnostic procedures.

One Kit, Two Applications- Accurate Results in Minutes

Flow CAST^® is intended for the determination of basophil activation in whole blood samples by flow cytometry. Our unique marker combination (CCR3 and CD63) combines the simple gating properties for basophil detection by CCR3 with the solid application of the proven activation marker CD63 in one test. Thanks to its easy protocol and smart marker combination the first results can be obtained in under an hour which provides perfect conditions for laboratories.

Features:

• Versatile and Robust: Solid applications for allergy testing and for screening in vitro and ex vivo studies
• Best Standardized Reagents for Basophil Activation: Optimized stimulation buffer, specific activators, and accurate quantification
• World-Class Support: Gold standard publications, excellent scientific/technical customer support
• QUICK: 30 minutes to quantified basophil activation inhibition

Kinase Inhibition Application
Simplifying Drug Development

BÜHLMANN’s Flow CAST Assay^® for Kinase Inhibition offers a quick and precise means to evaluate inhibitors of immune cell signaling pathway, using inhibition of basophil degranulation as a surrogate read out cell based system by flow cytometry.

Allergy Application
Allergy in a vial, resembling in vivo reactions to an allergen  

BÜHLMANN’s Flow CAST ^®Assay for Allergy provides an efficient method for cellular allergy stimulation testing using a broad range of quality controlled allergens and mixes which are standardized and validated for use in this assay and the CAST^® ELISA.

Relevant Resources

Relevant Resources