Vitamin B6 Enzymatic Assay is for Research Use Only. Not for diagnostic procedures in the US.
The assay is based on a three reagent protocol employing a PLP dependent enzyme and a following color reaction quantified in a photometer. The basic version runs in microtiter plates either by hand or pipetting robots without washing steps. The assay allows a batch-wise setting to quantify vitamin B6 and offers highly comparable results to HPLC and radioenzymatic assays.
Vitamin B6 is a water soluble compound found in many different foods, the biological active form, Pyridoxal 5’ phosphate (PLP) is essential for the function of more than 100 enzymes as a co-enzyme. Therefore signs of deficiency are manifold and severe states of deficiencies are seldom and mostly associated with deficiency of vitamin B12 and folate.
|Time to result
||45 min (approx.)
||9-250 nmol/L (2.2 – 62 ng/ml)
||EP 2 533 053 B1
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