Vitamin B6 Enzymatic Assay - BUHLMANN - Vitamin B6

Vitamin B6 Enzymatic Assay

Vitamin B6 Enzymatic Assay is for Research Use Only. Not for diagnostic procedures in the US.

Enzymatic Assay

The BÜHLMANN Vitamin B6 enzymatic assay (KK-VB6) is intended for the quantitative determination of Pyridoxal 5’- Phosphate (PLP, vitamin B6) in EDTA plasma.  The assay is based on a three reagent protocol employing a PLP dependent enzyme and a following color reaction quantified in a photometer. The basic version runs in microtiter plates either by hand or pipetting robots without washing steps. The assay allows a batch-wise setting to quantify vitamin B6 and offers highly comparable results to HPLC and radioenzymatic assays.


Vitamin B6

Vitamin B6 is a water soluble compound found in many different foods, the biological active form, Pyridoxal 5’ phosphate (PLP) is essential for the function of more than 100 enzymes as a co-enzyme. Therefore signs of deficiency are manifold and severe states of deficiencies are seldom and mostly associated with deficiency of vitamin B12 and folate.


Product Information

Method Enzymatic Assay
Time to result 45 min (approx.)
Sample type EDTA plasma
Calibrators 0.25-8.0 mg/ml
Reportable Range 9-250 nmol/L (2.2 – 62 ng/ml)
Package Size 100 tests
Patents EP 2 533 053 B1
Catalog Number see below

 Download IFU and Catalog Numbers

View the appropriate tab below for your location (US or Canada) and click on the blue underlined catalog number to view the Instructions for Use.