BÜHLMANN fCAL® turbo Calprotectin Assay Highlights

BÜHLMANN fCAL® turbo is for Research Use Only. Not to be used in diagnostic procedures.
Health Canada License: 96808

Unique in Speed, Quality, Flexibility

BÜHLMANN fCAL® turbo the turbidimetric immunoassay, is a flexible solution to be applied on most clinical chemistry analyzers. The technology implements a further milestone in fecal calprotectin quantification. It offers significant advantages over other competing methods in the market and is supported by publications in the field.


  • Speed: results within 10 minutes
  • Quality: This assay is standardized against the well established BÜHLMANN fCAL® ELISA and the PETIA methodolgy enhances precision and reproducibility.
  • Flexibility: Immunoturbiometric assays can be applied to most clinical chemistry platforms, allows random access workflow which can be directly integrated into the existing laboratory infrastructure.

Combined with the  CALEX®CAP extraction device, the fecal calprotectin workflow is further simplified thus reducing hands on time dramatically.

Advantages: BÜHLMANN fCAL® turbo

Proven Standardization

  • Based on partially purified human protein
  • More than 55 peer reviewed publications, use of grey zone proven in publications
  • Commutable results: fCAL® turbo to fCAL® ELISA (see data below)

Simplest Extraction Method in the Market

  • Pre-filled tubes: BÜHLMANN’s CALEX Cap® contains 5ml of stool extraction buffer in hygienic, closed system
  • Efficient Extraction: convenient, reliable sampling of stool using 1:500 dilution
  • Extract Stability:  3 days at RT, 18 months at -20°C

Speed: Short Analysis Time 

  • Analysis time of 10 minutes and TAT including extraction procedure is 30 minutes
  • Large Measuring Range: 20-8000 μg/g

Flexible and Individual Solutions

  • Applicable on most clinical chemistry analyzers
  • Large and growing list of validated protocols (see list below)
  • Safe Stool Extracts: Stool extracts can applied without any concern of system contamination (see data below)

Disadvantages: Competitor A’s Assay 

Poor Standardization

  • Based on recombinant protein
  • Only one study published, no grey zone proposed, high calprotectin levels tend to be underestimated.
  • Results lower than Competitor A’s ELISA and Unstable calibrator (6 hours at room temp)

Laborious extraction procedure

  • No pre-filled extraction devices
  • Extraction method (~45 minutes) requires multiple pipeting and other vortexing steps
  • Large differences in results between manual extraction and device extraction

Prolonged Time to First Result 

  • 3.5 times longer (system occupation): Time to first result is ~75 min (incl. extraction)
  • Limited measuring range: 5-800 µg/g

Limited Individual Solutions

  • Only applicable on DiaSorin LIAISON and LIAISON XL

canada Learn more about measuring range, CALEX®  Cap extraction device, and assay standardization for use in Canada.

Learn about safe stool extract application on clinical chemistry platforms with BÜHLMANN fCAL® turbo and CALEX® Cap .

united-states-of-america Learn more about measuring range, CALEX®  Cap extraction device, and assay standardization for use in the US.

Learn more about BÜHLMANN fCAL® turbo commutability to the BÜHLMANN fCAL® ELISA

Analyzer Validated Remarks
Siemens Advia 1650/1800 Siemens Advia XPT ††
Siemens Advia XPT †† new
Siemens Advia 2400
Siemens Dimension Vista 500/1500 establishment ongoing
Roche Cobas c501/c502 †††
Roche Cobas c701/702 †† new
Roche Cobas c111 ††
Roche Integra 800  †
Roche Cobas Integra 400+ validation ongoing
Abbott Architect (c series) ††
Beckman AU series †††
Beckman DxC 600/800 †† new
Mindray BS-380 ††
Mindray BS-200E †† new
Mindray BS-400 ††
Thermo Konelab30i ††
Thermo Indiko ††
Ortho Vitros 5600 ††
The Binding Site Optilite †† new
Horiba ABX Pentra 400 †† new
IDS iSYS †† new
††† optimized protocols available
†† validation data available
† application notes available

Contact Collin or Jennifer today  (Click on their email below) for more details on the BÜHLMANN fCAL® turbo automated calprotectin assay.

VP of Business Development


Technical Sales Specialist


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